Differential Expression of Benzophenone Synthase and Chalcone Synthase in Hypericum Sampsonii
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Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages
The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Im...
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Chalcone synthase (CHS) gene was amplified by PCR from cDNA of Solanum tuberosum leaf tissue. The gene was cloned into pGEMT easy vector and sequenced. It was then ligated into pET 30 vector to construct recombinant plasmid pET30-CHS. The recombinant was transformed into E.coli BL21 strain and the target gene was over expressed using IPTG. Optimum condition of IPTG concentration and temperature...
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Hypericum sampsonii Hance (Clusiaceae) is a folk medicine used in Taiwan to treat blood stasis, relieve swelling, and as an anti-hepatitis drug. Two new polyprenylated phloroglucinol derivatives, hypersampsone R (1) and hypersampsone S (2), and a known prenylated benzophenone, hyperibone K (3) were isolated from the aerial parts of H. sampsonii. Their structures were determined by extensive 1D ...
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Chenopodiaceae, Spinacia oleracea, Flavonoid Metabolism, Chalcone Synthase, Two Different Activities Chalcone synthase activity was found in enzyme preparations from spinach. In homogenates o f young leaves two different activities o f the enzyme could be separated by DEAE-ion exchange chromatography and chromatofocusing. Both activities formed naringenin with [2-14C] malonylCoA and 4-coumaroyl...
متن کاملCross-reaction of chalcone synthase and stilbene synthase overexpressed in Escherichia coli.
Chalcone synthase (CHS) and stilbene synthase (STS) are related plant polyketide synthases belonging to the CHS superfamily. CHS and STS catalyze common condensation reactions of p-coumaroyl-CoA and three C(2)-units from malonyl-CoA but different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. Using purified Pueraria lobata CHS and Arachis hypogaea STS overex...
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ژورنال
عنوان ژورنال: Natural Product Communications
سال: 2012
ISSN: 1934-578X,1555-9475
DOI: 10.1177/1934578x1200701219